C12FDG

C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than Fluorescein di (β-D-galactopyranoside) (HY-101895) for beta-galactosidase activity determinations in animal cells (Ex/Em = 488/523 nm) [1].

Product Specifications

CAS Number

[138777-25-0]

Product Name Alternative

5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/c12fdg.html

Purity

99.84

Solubility

DMSO : 50 mg/mL (ultrasonic)

Smiles

CCCCCCCCCCCC(NC1=CC2=C(C3(C4=C(OC5=C3C=CC(O[C@H]6[C@@H]([C@H]([C@H]([C@@H](CO)O6)O)O)O)=C5)C=C(O[C@H]7[C@@H]([C@H]([C@H]([C@@H](CO)O7)O)O)O)C=C4)OC2=O)C=C1)=O

Molecular Formula

C44H55NO16

Molecular Weight

853.90

Precautions

H302, H315, H319, H335

References & Citations

[1]Plovins A, et al. Use of fluorescein-di-beta-D-galactopyranoside (FDG) and C12-FDG as substrates for beta-galactosidase detection by flow cytometry in animal, bacterial, and yeast cells. Appl Environ Microbiol. 1994 Dec;60 (12) :4638-41.|[2]Udono M, et al. Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. Methods. 2012 Mar;56 (3) :383-8.