Anti-Ms Lime1 Purified
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Product Specifications
Certification
RUO
Reactivity
Mouse
Immunogen
Recombinant protein corresponding to amino acids 86-202 of mouse LIME
Target Antigen
Lime1
Clone
MLIME-05
Applications
IP, WB
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Other References
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
Applications Notes
Immunoprecipitation: Positive control: murine splenic T cells. Western blotting: Reducing conditions, recommended dilution: 1-5 μg/ml.
Isotype
Mouse IgG2a
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