Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Anti-Ms Lime1 Purified

LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.

Product Specifications

Certification

RUO

Reactivity

Mouse

Immunogen

Recombinant protein corresponding to amino acids 86-202 of mouse LIME

Target Antigen

Lime1

Clone

MLIME-05

Applications

IP, WB

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Other References

*Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107 (1) :213-21., URL: https://pubmed.ncbi.nlm.nih.gov/16160011/, *Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17 (1) :35-49., URL: https://pubmed.ncbi.nlm.nih.gov/15582487/, *Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4 (8) :603-16., URL: https://pubmed.ncbi.nlm.nih.gov/15286727/

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.

Applications Notes

Immunoprecipitation: Positive control: murine splenic T cells. Western blotting: Reducing conditions, recommended dilution: 1-5 μg/ml.

Isotype

Mouse IgG2a

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Citric Acid Anhydrous, ACS Grade
40300084-2 1 Kg

Citric Acid Anhydrous, ACS Grade

Ask
View Details
PTPRE Antibody
OAAN02178-50UL 50 µL

PTPRE Antibody

Ask
View Details
Frem1 (NM_001198811) Mouse Tagged ORF Clone
MR231925 10 µg

Frem1 (NM_001198811) Mouse Tagged ORF Clone

Ask
View Details
MAPK6PS2 Adenovirus (Human)
28065051 1.0 ml

MAPK6PS2 Adenovirus (Human)

Ask
View Details
Recombinant Human NTF3 Protein
E30P1520 20 µg

Recombinant Human NTF3 Protein

Ask
View Details
Mouse CMPK1 siRNA
abx912192-01 15 nmol

Mouse CMPK1 siRNA

Ask
View Details