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Anti-Hu p53 (pS392) Purified

The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.

Product Specifications

Certification

RUO

Immunogen

KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53.

Target Antigen

P53 (pS392)

Clone

FP3.2 [FPS392]

Applications

WB, IHC-P

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Blaydes JP, Craig AL, Wallace M, Ball HM, Traynor NJ, Gibbs NK, Hupp TR: Synergistic activation of p53-dependent transcription by two cooperating damage recognition pathways. Oncogene. 2000 Aug 10;19 (34) :3829-39., URL: https://www.ncbi.nlm.nih.gov/pubmed/10951576?dopt=, *Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15 (11) :909-912., URL: https://www.ncbi.nlm.nih.gov/pubmed/30377371 , *Koutová D, Havelek R, Peterová E, Muthná D, Královec K, Breiterová K, Cahlíková L, Řezáčová M: Pancracine, a montanine-type amaryllidaceae alkaloid, inhibits proliferation of A549 lung adenocarcinoma cells and induces apoptotic cell death in MOLT-4 leukemic cells. Int J Mol Sci. 2021 Jun 29;22 (13) :7014., URL: https://pubmed.ncbi.nlm.nih.gov/34209868/

Other References

*Tanigawa S, Fujii M, Hou DX: Stabilization of p53 is involved in quercetin-induced cell cycle arrest and apoptosis in HepG2 cells. Biosci Biotechnol Biochem. 2008 Mar;72 (3) :797-804., URL: https://www.ncbi.nlm.nih.gov/pubmed/18323654?ordin, *Taylor WR, Agarwal ML, Agarwal A, Stacey DW, Stark GR: p53 inhibits entry into mitosis when DNA synthesis is blocked. Oncogene. 1999 Jan 14;18 (2) :283-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/9927185?ordina, *Taylor WR, DePrimo SE, Agarwal A, Agarwal ML, Schönthal AH, Katula KS, Stark GR: Mechanisms of G2 arrest in response to overexpression of p53. Mol Biol Cell. 1999 Nov;10 (11) :3607-22., URL: https://www.ncbi.nlm.nih.gov/pubmed/10564259?ordin, *Agarwal ML, Agarwal A, Taylor WR, Chernova O, Sharma Y, Stark GR: A p53-dependent S-phase checkpoint helps to protect cells from DNA damage in response to starvation for pyrimidine nucleotides. Proc Natl Acad Sci U S A. 1998 Dec 8;95 (25) :14775-80., URL: https://www.ncbi.nlm.nih.gov/pubmed/9843965?ordina, *Agarwal ML, Agarwal A, Taylor WR, Stark GR: p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts. Proc Natl Acad Sci U S A. 1995 Aug 29;92 (18) :8493-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/7667317?ordina

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The mouse monoclonal antibody FP3.2 [FPS392] reacts with human p53 tumour suppressor intracellular protein phosphorylated at CKII site (Ser 392) .

Species

Human

CAS Number

9007-83-4

Isotype

Mouse IgG1

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