Anti-HLA-DR1 (empty) Purified

HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC) . The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.

Product Specifications

Certification

RUO

Reactivity

Human

Immunogen

Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.

Target Antigen

HLA-DR1 (empty)

Clone

MEM-267

Applications

WB, FC, ELISA

Concentration

1 mg/mL

Format

Purified

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Potolicchio I, Chitta S, Xu X, Fonseca D, Crisi G, Horejsi V, Strominger JL, Stern LJ, Raposo G, Santambrogio L: Conformational variation of surface class II MHC proteins during myeloid dendritic cell differentiation accompanies structural changes in lysosomal MIIC. J Immunol. 2005 Oct 15;175 (8) :4935-47. , URL: https://www.ncbi.nlm.nih.gov/pubmed/16210595?dopt=, *Carven GJ, Chitta S, Hilgert I, Rushe MM, Baggio RF, Palmer M, Arenas JE, Strominger JL, Horejsi V, Santambrogio L, Stern LJ: Monoclonal antibodies specific for the empty conformation of HLA-DR1 reveal aspects of the conformational change associated with peptide binding. J Biol Chem. 2004 Apr 16;279 (16) :16561-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/14757758?ordin

Storage Conditions

Store at 2-8°C. Do not freeze.

Specificity

The antibody MEM-267 specifically binds extracellularly to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages) .

Applications Notes

Flow cytometry: The antibody MEM-267 stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms.Western blotting: Recommended dilution: 1 µg/ml.