Anti-Por Acrosin Purified
Acrosin is a serine proteinase expressed in the acrosome of mature spermatozoa. This enzyme facilitates penetration of the sperm through the zona pellucida of the ovum.
Product Specifications
Certification
RUO
Negative Species
Dog,Cow,Human
Reactivity
Pig
Immunogen
Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Target Antigen
Acrosin
Clone
ACR-2
Applications
FC (QC tested), ICC, WB
Concentration
1 mg/mL
Format
Purified
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Peknicova J, Moos J. Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia. 1990 Sep-Oct;22 (5) :427-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/2073053?ordina, *Moos J, Tesarik J, Leca G, Peknicova J. Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin. FEBS Lett. 1991 Dec 2;294 (1-2) :27-30., URL: https://www.ncbi.nlm.nih.gov/pubmed/1743288?ordina, *Moos J, Peknicova J, Tesarik J. Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction. Biochim Biophys Acta. 1993 Apr 16;1176 (3) :199-207., URL: https://www.ncbi.nlm.nih.gov/pubmed/8471622?ordina, *Moos J, Peknicova J, Tesarik J. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod. 1993 Aug;49 (2) :408-15., URL: https://www.ncbi.nlm.nih.gov/pubmed/8373968?dopt=A, *Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M. Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro. Theriogenology. 2001 Jul 15;56 (2) :211-23. , URL: https://www.ncbi.nlm.nih.gov/pubmed/11480614?dopt=, *Ded L, Dostalova P, Dorosh A, Dvorakova-Hortova K, Peknicova J: Effect of estrogens on boar sperm capacitation in vitro. Reprod Biol Endocrinol. 2010 Jul 13;8:87, URL: http://www.ncbi.nlm.nih.gov/pubmed/20626847 , *Děd L, Dostálová P, Žatecká E, Dorosh A, Komrsková K, Pěknicová J: Fluorescent analysis of boar sperm capacitation process in vitro. Reprod Biol Endocrinol. 2019 Dec 19;17 (1) :109., URL: https://www.ncbi.nlm.nih.gov/pubmed/31856844
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
Applications Notes
Flow cytometry: Recommended dilution: 1-12 µg/ml. Intracellular staining.Immunocytochemistry: Membrane permeabilization (acetone) is essential.
Isotype
Mouse IgG1
Curated Selection
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