FLAG peptide

FLAG peptide is a multifunctional fusion tag for the purification of recombinant proteins. FLAG peptide maintances the natural folding of its fusing proteins. FLAG peptide can be removed by enterokinase, and eluted under non-denaturing conditions[1].

Product Specifications

CAS Number

[98849-88-8]

UNSPSC

12352209

Hazard Statement

H302, H315, H319, H335

Target

Biochemical Assay Reagents

Type

Peptides

Related Pathways

Others

Applications

COVID-19-immunoregulation

Field of Research

Inflammation/Immunology

Assay Protocol

https://www.medchemexpress.com/FLAG_peptide.html

Purity

99.23

Solubility

DMSO : 20 mg/mL (ultrasonic) |H2O : 100 mg/mL (ultrasonic)

Smiles

O=C(N[C@@H](CCCCN)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@H](C(O)=O)CCCCN)=O)=O)=O)=O)=O)[C@@H](NC([C@@H](N)CC(O)=O)=O)CC1=CC=C(O)C=C1

Molecular Formula

C41H60N10O20

Molecular Weight

1012.97

Precautions

H302, H315, H319, H335

References & Citations

[1]Einhauer A, et al. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49 (1-3) :455-65.|[2]Schuster M, et al. Protein expression in yeast; comparison of two expression strategies regarding proteinmaturation. J Biotechnol. 2000 Dec 28;84 (3) :237-48.|[3]Min-Joon Han, et al. Purification of functional reprogramming factors in mammalian cell using FLAG –Tag. Biochem Biophys Res Commun. 2017 Oct 14;492 (2) :154-160.