MYC-tag antibody
Product Specifications
Background
Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues (EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino-or carboxy-terminus of targeted proteins.
Synonyms
Myc tag
Host
Rabbit
Reactivity
Recombinant protein, Human
Immunogen
MYC-tag
Target
MYC-tag
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, WB, IP, IF
Purification
Immunogen affinity purified
Dilution
WB: 1:1000-1:8000; IP: 1:500-1:2000 ; IF: 1:10-1:100
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, WB, IP, IF
Immunogen Sequence
Peptide
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