Beta actin antibody
Product Specifications
Background
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle) . Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1 (found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.
Synonyms
Actin, cytoplasmic 1|Beta-actin|Actin, cytoplasmic 1, N-terminally processed|ACTB|β actin|β-actin
Gene ID
60
UniProt
P60709
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Actin, beta
Target
Beta actin
Clonality
Polyclonal
Isotype
IgG
Applications
ELISA, IF, WB, IHC, IP
Field of Research
Cell Division and Proliferation, Cardiovascular, Metabolism
Purification
Immunogen affinity purified
Dilution
WB: 1:500-1:5000; IHC: 1:20-1:500; IF: 1:50-1:500; IP: 1:500-1:2000
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
42 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Tested Applications
ELISA, IF, WB, IHC, IP
Curated Selection
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