Formamide

Formamide is an effective DNA denaturant that significantly reduces the stability of DNA in buffer solutions. Formamide can also be used as a decalcifying agent for rat cardiac cells. Additionally, formamide is widely used as a solvent or chemical raw material for ion compounds, resins, and plasticizers.[1][2][3][4][5][6][7]

Product Specifications

CAS Number

[75-12-7]

Product Name Alternative

Methanamide; Formimidic acid

UNSPSC

12352200

Hazard Statement

H351, H360, H373

Target

Biochemical Assay Reagents

Type

Biochemical Assay Reagents

Related Pathways

Others

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Cancer; Cardiovascular Disease

Assay Protocol

https://www.medchemexpress.com/Formamide.html

Purity

99.90

Solubility

DMSO : 100 mg/mL (ultrasonic) |H2O : 100 mg/mL (ultrasonic)

Smiles

O=CN

Molecular Formula

CH3NO

Molecular Weight

45.04

Precautions

H351, H360, H373

References & Citations

[1]Kamineni VK, et al. Layer-by-layer nanoassembly of polyelectrolytes using formamide as the working medium. Langmuir. 2007;23 (14) :7423-7.|[2]Blake RD, et al. Thermodynamic effects of formamide on DNA stability. Nucleic Acids Res. 1996 Jun 1;24 (11) :2095-103.|[3]Costanzo G, et al. Formamide as the main building block in the origin of nucleic acids. BMC Evol Biol. 2007 Aug 16;7 Suppl 2 (Suppl 2) :S1.|[4]Brette F, et al. Validation of formamide as a detubulation agent in isolated rat cardiac cells. Am J Physiol Heart Circ Physiol. 2002 Oct;283 (4) :H1720-8.|[5]Frankfurt OS, Krishan A. Identification of apoptotic cells by formamide-induced dna denaturation in condensed chromatin. J Histochem Cytochem. 2001 Mar;49 (3) :369-78.|[6]Messier PE.Messier PE. Effects of formamide on neuroepithelial cells and on interkinetic nuclear migration in the chick embryo. J Embryol Exp Morphol. 1976 Feb;35 (1) :197-212.|[7]National Toxicology Program. Toxicology and carcinogenesis studies of formamide (Cas No. 75-12-7) in F344/N rats and B6C3F1 mice (gavage studies) . Natl Toxicol Program Tech Rep Ser. 2008 Jul; (541) :1-192.