Calcein

Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein cannot directly cross the intact cell membrane of a living cell, unlike Calcein-AM (HY-D0041) which is cell-permeable. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems[1][2].

Product Specifications

CAS Number

[1461-15-0]

Product Name Alternative

Fluorexon

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H332, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Calcein.html

Solubility

1M NaOH : 50 mg/mL (ultrasonic) |DMSO : 2 mg/mL (ultrasonic) |H2O : 5.65 mg/mL (ultrasonic; warming; adjust pH to 8 with 1 M NaOH; heat to 60°C)

Smiles

O=C1OC2(C3=C(OC4=C2C=C(CN(CC(O)=O)CC(O)=O)C(O)=C4)C=C(O)C(CN(CC(O)=O)CC(O)=O)=C3)C5=C1C=CC=C5

Molecular Formula

C30H26N2O13

Molecular Weight

622.53

Precautions

H302, H315, H319, H332, H335

References & Citations

[1]Marques-Santos LF, et al. ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas. Fish Shellfish Immunol. 2017 Sep 5;70:195-203.|[2]Maherani B, et al., Calcein release behavior from liposomal bilayer; influence of physicochemical/mechanical/structural properties of lipids. Biochimie. 2013 Nov;95 (11) :2018-33.|[3]Shao B, et al. Caffeic Acid Phenyl Ester (CAPE) Protects against Iron-Mediated Cellular DNA Damage through Its Strong Iron-Binding Ability and High Lipophilicity. Antioxidants (Basel) . 2021 May 18;10 (5) :798.