Pyridostatin

Pyridostatin (RR82) is a G-quadruplex DNA stabilizing agent (Kd=490 nM) and can target DNA and RNA G4s in cells. Pyridostatin promotes growth arrest in human cancer cells by inducing replication- and transcription-dependent DNA damage. Pyridostatin targets the proto-oncogene Src. Pyridostatin reduced SRC protein levels and SRC-dependent cellular motility in human breast cancer cells[1][2].

Product Specifications

CAS Number

[1085412-37-8]

Product Name Alternative

RR82

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

G-quadruplex

Type

Reference compound

Related Pathways

Cell Cycle/DNA Damage

Applications

Cancer-programmed cell death

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/Pyridostatin.html

Purity

96.03

Solubility

DMSO : 100 mg/mL

Smiles

O=C(C1=CC(OCCN)=CC(C(NC2=NC3=C(C(OCCN)=C2)C=CC=C3)=O)=N1)NC4=CC(OCCN)=C5C=CC=CC5=N4

Molecular Formula

C31H32N8O5

Molecular Weight

596.64

Precautions

H302, H315, H319, H335

References & Citations

[1]Rodriguez R, et al. Small-molecule-induced DNA damage identifies alternative DNA structures in human genes. Nat Chem Biol. 2012;8 (3) :301-310. Published 2012 Feb 5.|[2]Koirala D, et al. A single-molecule platform for investigation of interactions between G-quadruplexes and small-molecule ligands. Nat Chem. 2011;3 (10) :782-787. Published 2011 Aug 28.|[3]Moruno-Manchon JF, Koellhoffer EC, Gopakumar J, et al. The G-quadruplex DNA stabilizing drug pyridostatin promotes DNA damage and downregulates transcription of Brca1 in neurons. Aging (Albany NY) . 2017;9 (9) :1957-1970.|[4]Zhang X, et al. Chemical profiling of DNA G-quadruplex-interacting proteins in live cells. Nat Chem. 2021 Jul;13 (7) :626-633.