OCT3 Colorimetric Cell-Based ELISA Kit

The OCT3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor OCT3 protein expression profile in cells. The kit can be used for measuring the relative amounts of OCT3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on OCT3.

Product Specifications

Synonyms

POU domain class 5 transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3; POU5F1; OCT3; OCT4; OTF3

Gene Name

POU5F1

UniProt

Q01860

Reactivity

Human, Mouse, Rat

Tissue Specificity

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Applications

ELISA

Sample Type

Cell lines

Detection Range

> 5000 cells/well

Function

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') . Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.