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Anti-MLKL Rabbit Monoclonal Antibody

Boster Bio Anti-MLKL Rabbit Monoclonal Antibody catalog # M00535. Tested in WB, IHC applications. This antibody reacts with Human.

Product Specifications

Synonyms

Mixed lineage kinase domain-like protein; hMLKL; MLKL

Gene Name

Mixed lineage kinase domain-like protein

UniProt

Q8NB16

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human MLKL

Clonality

Monoclonal

Clone

Clone: DGB-13

Tissue Specificity

Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level) . Isoform 3 is expressed in vascular smooth muscle cells (VSMC) . .

Applications

WB, IHC

Field of Research

Cancer, Cell Death, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=M00535

Product MSDS

https://www.bosterbio.com/msds?sku=M00535

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 50-200

Other Gene Names

MLKL

Subcellular Location

Cytoplasm . Cell membrane . Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction.

Protein Name

T-cell surface glycoprotein CD4

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