Anti-GATA4 Monoclonal Antibody

GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.

Cancer, Cardiovascular, Developmental Biology, Domain Families, Epigenetics and Nuclear Signaling, Hypertrophy, Lineage Markers, Lineage Specification, Mesenchymal Stem Cells, Oncoproteins/Suppressors, Stem Cells, Transcription, Transcription Factors, Tumor Suppressors, Zinc Finger

Anti-GATA4 was purified from concentrated tissue culture supernatant Protein A chromatography. A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93% homology. Cross-reactivity with GATA4 from other sources has not been determined.

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169) . Involved in bone morphogenetic protein (BMP) - mediated induction of cardiac-specific gene expression (By similarity) . Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity) . Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity) . Promotes cardiac myocyte enlargement (PubMed:20081228) . Required during testicular development (PubMed:21220346) . May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735) .

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Anti-GATA4 antibody has been tested by ELISA, SDS-Page, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysate or extract.