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Anti-MIF Rabbit Monoclonal Antibody

Boster Bio Anti-MIF Rabbit Monoclonal Antibody catalog # M00411. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Macrophage migration inhibitory factor; MIF;5.3.2.1; Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase;5.3.3.12; Phenylpyruvate tautomerase; MIF; GLIF, MMIF

Gene Name

Macrophage migration inhibitory factor

UniProt

P14174

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human MIF

Clonality

Monoclonal

Clone

Clone: ABAG-13

Tissue Specificity

Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.

Applications

WB, IP, Flow Cytometry

Field of Research

Immunology, Innate Immunity, Macrophage/Inflammation

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti- inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=M00411

Product MSDS

https://www.bosterbio.com/msds?sku=M00411

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IP 1:50<br>FC 1:50

Other Gene Names

MIF

Subcellular Location

Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non- classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.

Protein Name

T-cell surface glycoprotein CD4

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