Anti-IKK beta IKBKB Antibody

NFkB comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death. In the cytoplasm NFkB is negatively modulated by the inhibitory proteins IkB. In turn IkB is phosphorylated by a cellular kinase complex called IKK. IKK is a heterodimer composed of two kinases: IKK-a and IKK-b that phosphorylate IkB leading to its degradation and the resulting translocation of NFkB to the nucleus. IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF- a, endotoxins and overexpression of cellular kinases like MEKK1. Aspirin appears to have its effect by inhibiting the binding of ATP to IKK.

IKK beta, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IkBKB, IKK-beta, IKK-B, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB

Atherosclerosis, Cardiovascular, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, NFKB Pathway, Nuclear Signaling Pathways, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction, TLR Signaling, Vascular Inflammation

This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-IKKb may react non-specifically with other proteins. Control peptide (code #100-401-220p) will compete only with the specific reaction of antiserum with the IKKb subunit.

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

This product was tested by immunoblot and found to be reactive against IKK-b at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . Anti-IKKb is suitable for the detection by immunoblot of human, mouse and rat IKKb showing an 87 kDa band. Anti-IKKb has been tested in IHC using human placenta tissue.