Mouse ALCAM/CD166 ELISA Kit PicoKine®

CD166 antigen is a 100-105 kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.

Detected on brain motor neurons, in differentiating retinal ganglion cells and in adult retina (PubMed:15345243) . Detected on leukocytes and on lymphatic endothelial cells (PubMed:23169771) . Detected in spleen B cells and T-cells (at protein level) (PubMed:9209500) . Detected in adult brain and embryonic spinal cord (PubMed:15345243) . Expressed at high levels in the brain, and lung, and at lower levels in the liver, and the kidney, as well as by activated leukocytes (PubMed:9209500) . .

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6 (By similarity) . Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (By similarity) . Mediates homotypic interactions with cells that express ALCAM (PubMed:24740813) . Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction. Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed:23169771) . Required for normal organization of the lymph vessel network (PubMed:23169771) . Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed:24740813) . Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow (PubMed:25730656) . Promotes in vitro osteoblast proliferation and differentiation (PubMed:25730656) . Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM (By similarity) . Mediates outgrowth and pathfinding for retinal ganglion cell axons (PubMed:15345243) . .

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell membrane ; Single-pass type I membrane protein . Cell projection, axon . Cell projection, dendrite . Detected at the immunological synapse, i.e, at the contact zone between antigen-presenting dendritic cells and T-cells. Colocalizes with CD6 and the TCR/CD3 complex at the immunological synapse. .