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Anti-Vimentin Antibody (Monoclonal, V9)

Boster Bio Anti-Vimentin Antibody (Monoclonal, V9) catalog # MA1102. Tested in IF, IHC, WB applications. This antibody reacts with Human, Pig, Rabbit, Rat.

Product Specifications

Background

The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.

Synonyms

Vimentin; Vim

Gene Name

Vimentin

UniProt

P31000

Host

Mouse

Reactivity

Human, Pig, Rabbit, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

Pig eye lens vimentin.

Clonality

Monoclonal

Clone

Clone: V9

Tissue Specificity

VIM: Highly expressed in fibroblasts, someexpression in T- and B-lymphocytes, and little or no expression inBurkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

Applications

IF, IHC, WB

Field of Research

Neuroscience, Signal Transduction, Stem Cells, Tumor Biomarkers

Purification

Ascites

Concentration

Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.

Form

Lyophilized

Reconstitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Function

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. .

Storage Conditions

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.bosterbio.com/datasheet?sku=MA1102

Product MSDS

https://www.bosterbio.com/msds?sku=MA1102

Fragment

Mouse IgG1

Applications Notes

Western blot, 0.5-1μg/ml, Human, rabbit, pig, rat<br> Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, rabbit, pig, rat, By Heat<br> Immunofluorescence, 5μg/ml, Human

Other Gene Names

VIM

Subcellular Location

Nucleus matrix . Cytoplasm .

Sequence Similarities

Belongs to the intermediate filament family.

Protein Name

Vimentin

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