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Rat EGF ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Rat EGF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development) . Quantitate Rat EGF in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Specifications

Background

Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.

Synonyms

Pro-epidermal growth factor; EGF; Epidermal growth factor; Egf

Gene Name

Epidermal growth factor

UniProt

P07522

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: N974-R1026

Applications

ELISA

Field of Research

Growth Factors/Hormones, Signal Transduction

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

7.8 pg/ml - 500 pg/ml

Function

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity) .

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Product Datasheet

https://www.bosterbio.com/datasheet?sku=EZ0954

Product MSDS

https://www.bosterbio.com/msds?sku=EZ0954

Other Gene Names

EGF

Subcellular Location

Membrane, Single-pass type I membrane protein

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