Anti-Dicer Antibody

Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes (By similarity). {UniProtKB:Q9UPY3}.; [Isoform 2]: More active than isoform 1 to process long double-stranded RNA into siRNAs. Responsible for the accumulation of endogenous siRNAs observed in mouse oocytes compared to somatic cells and it regulates meiotic spindle organization in female germline.

Immunoblotting: use at 1ug/mL. Predicted molecular weight is ~215kDa. <br><br>Positive control: rat brain lysate. <br><br>These are recommended concentrations. <br><br>Endusers should determine optimal concentrations for their applications.

Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs). After long primary transcript pri-miRNAs are processed to stem-looped pre- miRNAs by Drosha, pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22- nucleotide mature miRNAs . The mature miRNA then becomes a part of the RNA- Induced Silencing Complex (RISC) and can bind to the 3′ UTR of the target mRNA.