Evans Blue

Evans Blue (Direct Blue 53) is a potent inhibitor of L-glutamate uptake via the membrane bound excitatory amino acid transporter (EAAT) . Evans Blue is a L-glutamate and kainate receptor-mediated currents inhibitor. Evans Blue has a strong affinity towards serum albumin, making it a high molecular weight protein tracer. Evans Blue is also used to study BBB (blood-brain barrier) permeability[1][2].

Product Specifications

CAS Number

[314-13-6]

Product Name Alternative

Direct Blue 53; T-1824; C.I. 23860

UNSPSC

12352200

Hazard Statement

H350

Target

EAAT; iGluR

Type

Biochemical Assay Reagents

Related Pathways

Membrane Transporter/Ion Channel; Neuronal Signaling

Field of Research

Neurological Disease; Cardiovascular Disease

Assay Protocol

https://www.medchemexpress.com/Evans-Blue.html

Purity

99.86

Solubility

DMSO : ≥ 25 mg/mL|H2O : 31.25 mg/mL (ultrasonic)

Smiles

CC1=CC(C2=CC=C(/N=N/C3=C(O)C4=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C4C=C3)C(C)=C2)=CC=C1/N=N/C5=C(O)C6=C(N)C(S(=O)(O[Na])=O)=CC(S(=O)(O[Na])=O)=C6C=C5

Molecular Formula

C34H24N6Na4O14S4

Molecular Weight

960.81

Precautions

H350

References & Citations

[1]Anatol Manaenko, et al. Comparison Evans Blue injection routes: Intravenous versus intraperitoneal, for measurement of blood-brain barrier in a mice hemorrhage model. J Neurosci Methods. 2011 Feb 15;195 (2) :206-10. |[2]Linpeng Yao, et al. Evans Blue Dye: A Revisit of Its Applications in Biomedicine. Contrast Media Mol Imaging. 2018 Apr 22:2018:7628037.