Alpha-2-M Antibody / Alpha-2-Macroglobulin / A2M

A2-Macroglobulin acts as an antiprotease and is able to inactivate an enormous variety of proteinases. It functions as an inhibitor of fibrinolysis by inhibiting plasmin and kallikrein. It functions as an inhibitor of coagulation by inhibiting thrombin. A2-macroglobulin may act as a carrier protein because it also binds to numerous growth factors and cytokines, such as platelet-derived growth factor, basic fibroblast growth factor, TGF-b, insulin, and IL-1b. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P01023

Host

Mouse

Reactivity

Human

Immunogen

A portin of amino acids 604-748 from the human protein was used as the immunogen for the Alpha-2-M antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

A2M/3622

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the Alpha-2-M antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Alpha-2-M antibody is available for research use only.

Storage Conditions

Aliquot the Alpha-2-M antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the Alpha-2-M antibody should be determined by the researcher.

Location

Secreted in plasma

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using Alpha-2-M antibody (clone A2M/3622) . These results demonstrate the foremost specificity of the A2M/3622 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.