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Recombinant PAX7 Antibody

The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma) .

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P23759

Host

Mouse

Reactivity

Human

Immunogen

A recombinant fragment (amino acids 301-505) of human PAX7 protein was used as the immunogen for the recombinant PAX7 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG2b κ

Clone

RPAX7/9325

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant PAX7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant PAX7 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant PAX7 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/recombinant-pax7-antibody-rpax79325-v5518

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant PAX7 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

SDS-PAGE analysis of purified, BSA-free recombinant PAX7 antibody (clone rPAX7/9325) as confirmation of integrity and purity.

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