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SETD3 Antibody / Actin-histidine N-methyltransferase

SET domain containing 3 (SETD3) is a protein that in humans is encoded by the SETD3 gene. SETD3 is a histone methyltransferase. In mouse muscle cells, Setd3 catalyzes mono- and dimethylation of histone H3 lysine-4 and H3K36 and promotes transcription of genes required for muscle cell differentiation.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q86TU7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids Q173-R468) was used as the immunogen for the SETD3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the SETD3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SETD3 antibody is available for research use only.

Storage Conditions

After reconstitution, the SETD3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Product Datasheet

https://www.nsjbio.com/tds-pdf/setd3-antibody-actin-histidine-n-methyltransferase-rq7583

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SETD3 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

Western blot testing of 1) rat heart, 2) rat H9c2 (2-1), 3) rat C6, 4) mouse heart, 5) mouse C2C12 and 6) mouse NIH 3T3 cell lysate with SETD3 antibody. Predicted molecular weight ~67 kDa.
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