PU.1 Antibody

Transcription factor PU.1 binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500

UniProt

P17947

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human SPI1/PU.1 was used as the immunogen for the PU.1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

1835CT291.10.52

Applications

Purity

Protein G purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PU.1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PU.1 antibody is available for research use only.

Storage Conditions

Aliquot the PU.1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PU.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of PU.1 antibody at 1:500: Lane 1) human K562, 2) Daudi and 3) THP1 cell lysate. Predicted molecular weight ~31 kDa but routinely observed at ~40 kDa.