RGS19 (phospho-S151) polyclonal antibody

Product Specifications

Background

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. In mammals, G protein α, β and γ polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their α subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four Gα GTPase-activating proteins (GAPs) have been identified and are designated RGS1 (regulator of G protein signaling), RGS4, RGS10 and GAIP (Gα-interacting protein) . Each of these proteins has been shown to deactivate specific Gα isoforms by increasing the rate at which they convert GTP to GDP, RGS1, RGS4 and GAIP bind tightly to and exhibit GAP activity towards Gα i , Gα o and Gα t , but not Gα s. RGS10 increases the GTP hydrolytic activity of several members of the Gα i subfamily including Gα i-3, Gα z and Gα o.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Regulator of G-protein signaling 19; RGS19; G-alpha-interacting protein; GAIP; RGS19; GAIP; GNAI3IP

Swiss Prot

P49795

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human RGS19 around the phosphorylation site of Serine 151.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 25 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

RGS19 (phospho-S151) polyclonal antibody detects endogenous levels of RGS19 protein only when phosphorylated at Ser151.

Applications Notes

Western blot (WB) analysis of RGS19 (phospho-S151) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:H1792 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug)