COX8A polyclonal antibody

Product Specifications

Background

The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water and ATP. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. The COX8 (cytochrome c oxidase subunit VIII) subunits are nuclear and have muscle and non-muscle-specific isoforms. COX8 exists as three isoforms: COX8a, a liver and heart isoform, Cox8b, a heart-specific isoform, and Cox8c, whose expression pattern has yet to be elucidated. All three Cox8 isoforms exists as components of the COX complex and play an important role in electron transport.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Cytochrome c oxidase subunit 8A; mitochondrial; Cytochrome c oxidase polypeptide VIII-liver/heart; Cytochrome c oxidase subunit 8-2; COX8A; COX8, COX8L

Swiss Prot

P10176

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human COX8A.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 8 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

COX8A polyclonal antibody detects endogenous levels of COX8A protein.

Applications Notes

Western blot (WB) analysis of COX8A polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:Rat brain tissue lysate