FucT-III polyclonal antibody

Product Specifications

Background

Glycosyltransferases that mediate the regio- and stereoselective transfer of sugars, such as the fucosyltransferases, determine cell surface-carbohydrate profiles, which is an essential interface for biological recognition processes. Fucosyltransferases catalyze the covalent association of fucose to different positional linkages in sugar acceptor molecules. The carbohydrate moieties generated and covalently attached to cell surfaces are necessary to ensure a surface contour that satisfies physiological roles, which are reliant on adhesion molecules such as Selectin.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Galactoside 3 (4) -L-fucosyltransferase; Blood group Lewis alpha-4-fucosyltransferase; Lewis FT; Fucosyltransferase 3; Fucosyltransferase III; FucTIII; FucT-3; FucT3; FUT3; FT3B; LE

Swiss Prot

P21217

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 138-183 of Human FucT-III.

Conjugation

Unconjugated

Applications

WB IHC ICC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 42 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

FucT-III polyclonal antibody detects endogenous levels of FucT-III protein.

Applications Notes

Western blot (WB) analysis of FucT-III polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate Lane2:Raw264.7 cell lysate Lane3:PC12 cell lysate