AQP5 (Y243) polyclonal antibody

Product Specifications

Background

Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation, and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. In the lung, AQP5 is responsible for the majority of water transport across the apical membrane of type I alveolar epithelial cells.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Aquaporin-5; AQP-5; Aquaporin5; AQP5; Aquaporin 5; AQP 5

Swiss Prot

P55064

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 220-270 of Human AQP5.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 36 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

AQP5 (Y243) polyclonal antibody detects endogenous levels of AQP5 protein.

Applications Notes

Western blot (WB) analysis of AQP5 (Y243) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (20ug) Lane3:PC12 whole cell lysate (40ug)