Plakophilin 2 (P663) polyclonal antibody

Product Specifications

Background

Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress) . Plakophilin 1 mediates increases in desmosomal protein content, desmosome assembly and regulation of cell migration. Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart. Plakophilin 4 is a component of desmosomal adhesion plaques that regulates junctional plaque organization and cadherin function.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Plakophilin-2; PKP2

Swiss Prot

Q99959

Reactivity

Human, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 634-680 of Human Plakophilin 2.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 97 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Plakophilin 2 (P663) polyclonal antibody detects endogenous levels of Plakophilin 2 protein.

Applications Notes

Western blot (WB) analysis of Plakophilin 2 (P663) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate (20ug) Lane2:HCT116 whole cell lysate (20ug) Lane3:The Heart tissue lysate of Rat (20ug)