CYB5R3 (I169) polyclonal antibody

Product Specifications

Background

CYB5R3 (NADH-cytochrome b5, reductase 3, diaphorase 1) is a 301 amino acid protein encoded by the human gene CYB5R3. CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and has two naturally occuring isoforms. Isoform 1 is anchored to the cytoplasmic side of the endoplasmic reticulum membrane and mitochondrion outer membrane, while isoform 2 is the soluble form found in erythrocytes. CYB5R3 is involved in the desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism and, in erythrocytes, methemoglobin reduction.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; Diaphorase-1; Diaphorase1; Diaphorase 1; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form; DIA1

Swiss Prot

P00387

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 141-190 of Human CYB5R3.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 34 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CYB5R3 (I169) polyclonal antibody detects endogenous levels of CYB5R3 protein.

Applications Notes

Western blot (WB) analysis of CYB5R3 (I169) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:The Testis tissue lysate of Rat (40ug) Lane4:The Testis tissue lysate of Mouse (40ug)