HnRNP K (Y280) polyclonal antibody

Product Specifications

Background

The majority of hnRNP proteins components are localized to the nucleus; however some shuttle between the nucleus and the cytoplasm, such as hnRNP K. hnRNP K, a 65 kDa protein, recruits a variety of molecular partners through two K homologous (KH) domains, which are required for protein-protein interactions. hnRNP K also contains several potential phosphorylation sites, including Ser 302, the major site of PKCd phosphorylation, which are thought to regulate various cellular functions, including sequence-specific DNA binding, transcription, RNA binding and nucleocytoplasmic shuttling.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Heterogeneous nuclear ribonucleoprotein K; hnRNP K; Transformation up-regulated nuclear protein; TUNP; HNRNPK; HNRPK

Swiss Prot

P61978

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 250-300 of Human hnRNP K.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 58-62 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HnRNP K (Y280) polyclonal antibody detects endogenous levels of hnRNP K protein.

Applications Notes

Western blot (WB) analysis of hnRNP K (Y280) pAb at 1:1000 dilution Lane1:HCC827 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (20ug) Lane3:A549 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug)