Ang-1 (E207) polyclonal antibody

Product Specifications

Background

Angiopoietin-1 (Ang-1) is a secreted ligand for Tie-2. Preliminary biochemical analyses of Ang-1 reveal a potential Fibrinogen-like domain at the carboxy-terminus and coiled-coil regions in the amino-terminus. Ang-1 is an angiogenic factor that is thought to be involved in endothelial development. A related protein, angiopoietin-2 (Ang-2), has been identified as a naturally occurring antagonist of Ang-1 activation of Tie-2. In adult tissue, Ang-2 expression seems to be restricted to sites of vascular remodeling.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Angiopoietin-1; ANG-1; KIAA0003; ANGPT1

Swiss Prot

Q15389

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 176-224 of Human Ang-1.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 58kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Ang-1 (E207) polyclonal antibody detects endogenous levels of Ang-1 (E207) protein.

Applications Notes

Western blot (WB) analysis of Ang-1 (E207) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate (40ug) Lane2:NIH-3T3 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug)