NGFR p75 (P150) polyclonal antibody

Product Specifications

Background

NGFR p75 is a 75kD transmembrane glycoprotein (399 a.a.) consisting of an extracellular domain (222 a.a.) which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain (22 a.a.), and a cytoplasmic domain (155 a.a.) . NGFR p75 is mainly expressed in Schwann cells and neurons and in a variety of non neuronal cells. NGFR p75 is necessary for regulating neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tumor necrosis factor receptor superfamily member 16; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; NGF receptor; p75 ICD; CD271; NGFR; TNFRSF16

Swiss Prot

P08138

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 111-160 of Human NGFR p75.

Conjugation

Unconjugated

Related Pathways

Neuronal and Glial Cell Marker Atlas

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 45,75 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

NGFR p75 (P150) polyclonal antibody detects endogenous levels of NGFR p75 protein.

Applications Notes

Western blot (WB) analysis of CD271/NGFR p75 (P150) at 1:500 dilution Lane1:A2780 whole cell lysate (20ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:3T3-L1 whole cell lysate (20ug)