HnRNP C1/2 (D271) polyclonal antibody

Product Specifications

Background

The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA) . These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. hnRNP C1 and C2 are encoded by one gene; the two alternatively spliced transcript variants have been described.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2; HNRNPC; HNRPC

Swiss Prot

P07910

Reactivity

Human, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 241-290 of Human hnRNP C1.

Conjugation

Unconjugated

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 33 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HnRNP C1/2 (D271) polyclonal antibody detects endogenous levels of hnRNP C1/2 protein.

Applications Notes

Western blot (WB) analysis of hnRNP C1/C2 (D271) pAb at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (20ug) Lane4:K562 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug)