CaMKIIβ/γ (R631) polyclonal antibody

Product Specifications

Background

The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated α, β, γ and δ, which may or may not be coexpressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit gamma; CaM kinase II subunit gamma; CaMK-II subunit gamma; CAMK2G; CAMK; CAMK-II; CAMKG

Swiss Prot

Q13555/Q13554

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 601-652of Human CaMKIIβ.

Conjugation

Unconjugated

Related Pathways

Phospholipase Signaling, Phospholipase Signaling, Contribution of Soluble Factors to EMT, G Protein-coupled Receptors Signaling to MAPK Erk, Mitochondrial Control of Apoptosis, ErbB HER Signaling

Applications

WB IHC IF

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 50~80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CaMKIIβ/γ (R631) polyclonal antibody detects endogenous levels of CaMKIIβ and CaMKIIγ protein.

Applications Notes

Western blot (WB) analysis of CaMKIIβ/γ (R631) pAb at 1:500 dilution Lane1:U-87MG whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug) Lane5:CT26 whole cell lysate (40ug)