NF-YB (Q30) polyclonal antibody

Product Specifications

Background

The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA-binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein, histone-fold “handshake” motif in a manner analogous to the histone proteins H2B and H2A, respectively.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B; NF-YB; NFYB; HAP3

Swiss Prot

P25208

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to the N-terminal of Human NF-YB.

Conjugation

Unconjugated

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 32 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

NF-YB (Q30) polyclonal antibody detects endogenous levels of NF-YB protein.

Applications Notes

Western blot (WB) analysis of NF-YB (Q30) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Rat (40ug) Lane2:SGC7901 whole cell lysate (40ug) Lane3:A375 whole cell lysate (40ug) Lane4:Panc1 whole cell lysate (40ug)