AKT1 (M118) polyclonal antibody

Product Specifications

Background

AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold; overexpression or inappropriate activation of AKT has been seen in many types of cancer.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα

Swiss Prot

P31749

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 90-130 of Human AKT1.

Conjugation

Unconjugated

Related Pathways

Phosphoinosotode Signaling, PI3K AKT signaling, Protein Kinase C Signaling, Warburg Effect, T cell receptor signaling, Inhibition of Apoptosis, Insulin &Glucose Signaling, Cell Cycle Control G1 S Checkpoint, Adherens Junction signaling, Mitochondrial Control of Apoptosis, MTOR signaling, ErbB HER Signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

AKT1 (M118) polyclonal antibody detects endogenous levels of AKT1 protein, this antibody does not cross-react with AKT2 or AKT3.

Applications Notes

Western blot (WB) analysis of Akt1 (M118) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:The Lung tissue lysate of Rat (20ug) Lane4:3T3-L1 whole cell lysate (40ug)