PAK4/5/6 (V469) polyclonal antibody
Product Specifications
Background
PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction.PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Serine/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4; PAK4; KIAA1142; Serine/threonine-protein kinase PAK 6; PAK-5; p21-activated kinase 6; PAK-6; PAK6; PAK5; Serine/threonine-protein kinase PAK 7; p21-activated kinase 5; p21-activated kinase 7; PAK-7; PAK7; KIAA1264
Swiss Prot
O96013/Q9NQU5/Q9P286
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 441-490 of Human PAK4.
Conjugation
Unconjugated
Applications
WB IP
Dilution
WB: 1:500~1:1000 IP: 1:10~1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 64 kDa (PAK4), 80 kDa (PAK5), 75 kDa (PAK6)
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PAK4/5/6 (V469) polyclonal antibody detects endogenous levels of PAK4, PAK5 and PAK6 protein.
Applications Notes
Western blot (WB) analysis of PAK4/5/6 (V469) pAb at 1:500 dilution Lane1:H1792 whole cell lysate (20ug) Lane2:MCF-7 whole cell lysate (20ug) Lane3:PC3 whole cell lysate (20ug) Lane4:The Testis tissue lysate of Mouse (40ug) Lane5:The Testis tissue lysate of Rat (40ug)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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