TBK1 (Phospho-S172) polyclonal antibody

Product Specifications

Background

The transcription factor NFκB is retained in the cytoplasm in an inactive form by the inhibitory protein IκB. Activation of NFκB requires that IκB be phosphorylated on specific serine residues, which results in targeted degradation of IκB. IκB kinase α (IKKα), previously designated CHUK, interacts with IkB-a and specifically phosphorylates IκB-α on the sites that trigger its degradation, serines 32 and 36. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IκB phosphorylation. TANK binding kinase (TBK1), also designated T2K, is a novel IKK-related kinase that has been identified in murine and human tissues. TBK1 was shown to complex with TRAF2 and TANK in the NFκB activation pathway. TBK1 shares homology with IKKα and IKKβ in the amino-terminal half, which includes the kinase domain.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Serine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; T2K; TANK-binding kinase 1; TBK1; NAK

Swiss Prot

Q9UHD2

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human TBK1 around the phosphorylation site of Serine 172.

Conjugation

Unconjugated

Related Pathways

NF-kB Signaling, Immune Cell Signaling, STING Signaling

Applications

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 100 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TBK1 (Phospho-S172) polyclonal antibody detects endogenous levels of TBK1 protein only when phosphorylated at Ser172.

Applications Notes

Western blot (WB) analysis of TBK1 (Phospho-S172) polyclonal antibody at 1:500 dilution LaneA:The Testis tissue lysate of Mouse LaneB:The Testis tissue lysate of Rat LaneC:U-87MG whole cell lysate