PINK1 (Phospho-S228) polyclonal antibody

Product Specifications

Background

PTEN induced putative kinase 1, PINK1, is a mitochondrial serine/threonine kinase involved in the normal function and integrity of mitochondria, as well as in reduction of cytochrome c release from mitochondria. PINK1 phosphorylates Parkin and promotes its translocation to mitochondria. Research studies have shown that mutations in PINK1 are linked to autosomal recessive early onset Parkinson’s disease, and are associated with loss of protective function, mitochondrial dysfunction, aggregation of α-synuclein, as well as proteasome dysfunction.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Serine/threonine-protein kinase PINK1, mitochondrial; BRPK; PTEN-induced putative kinase protein 1; PINK1

Swiss Prot

Q9BXM7

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human PINK1 around the phosphorylation site of Serine 228.

Conjugation

Unconjugated

Applications

Dilution

WB:1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PINK1 (Phospho-S228) polyclonal antibody detects endogenous levels of PINK1 protein only when phosphorylated at Ser228.

Applications Notes

Western blot (WB) analysis of PINK1 (Phospho-S228) polyclonal antibody at 1:500 dilution LaneA:The Testis tissue lysate of Mouse LaneB:The Testis tissue lysate of Rat LaneC:HEK293T whole cell lysate LaneD:MCF-7 whole cell lysate LaneE:U-87MG whole cell lysate