MEK-7 (phospho Ser271) Polyclonal Antibody

Product Specifications

Background

Mitogen-activated protein kinase kinase 7 (MAP2K7) Homo sapiens The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Product Name Alternative

MAP2K7; JNKK2; MEK7; MKK7; PRKMK7; SKK4; Dual specificity mitogen-activated protein kinase kinase 7; MAP kinase kinase 7; MAPKK 7; JNK-activating kinase 2; MAPK/ERK kinase 7; MEK 7; Stress-activated protein kinase kinase 4; SAPK kinase 4; S

Swiss Prot

O14733

Reactivity

Human, Mouse, Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human MAP2K7 around the phosphorylation site of Ser271. AA range:236-285

Conjugation

Unconjugated

Related Pathways

SAPK JNK Signaling Cascades

Applications

WB, IHC-p, IF (paraffin section), ELISA

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Modification

Phosphorylation

Molecular Weight

~47 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-MEK-7 (S271) Polyclonal Antibody detects endogenous levels of MEK-7 protein only when phosphorylated at S271.

Applications Notes

Western Blot analysis of 3T3 cells using Phospho-MEK-7 (S271) Polyclonal Antibody

Host or Source

Rabbit

Available Sizes

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MBS7052933-01 0.05 mg

CASC1 Antibody

Ask

View Details

MBS7052933-02 0.1 mg

CASC1 Antibody

Ask

View Details

MBS7052933-03 5x 0.1 mg

CASC1 Antibody

Ask

View Details

E0542 1 Unit

Three Holes Water Bath Boiler; Water Bath; Thermostat Water Bath

Ask

View Details

033486-ML405 100 µL

CD274, CT (CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, Programmed cell death 1 ligand 1, B7 homolog 1, CD274) (MaxLight 405) discontinued

Ask

View Details

RC217443L1 10 µg

Bile Acid Receptor (NR1H4) (NM_005123) Human Tagged Lenti ORF Clone

Ask

View Details