SMC6 Polyclonal Antibody

Product Specifications

Background

SMC5/6 complex is recruited to nuclease induced double strand breaks (DSBs) and is required for the recruitment of cohesin to DSBs. The human SMC5/6 complex and the SUMO ligase activity of hMMS21 are required for the prevention of DNA damage induced apoptosis by facilitating DNA repair in human cells.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

SMC6L1; SMC 6; SMC6; SMC6 protein; SMC6 structural maintenance of chromosomes 6 like 1; Structural maintenance of chromosomes 6; Structural maintenance of chromosomes 6 like 1; Structural maintenance of chromosomes protein 6.

Swiss Prot

Q96SB8

Reactivity

Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep

Immunogen

KLH conjugated synthetic peptide derived from human SMC6:155-260/1091

Conjugation

Unconjugated

Applications

IHC-P

Dilution

IHC-P=1:100-500

Purity

Affinity purified by Protein A

Modification

Unmodification

Molecular Weight

126 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

SMC6 Polyclonal Antibody detects endogenous levels of SMC6 protein.

Applications Notes

Paraformaldehyde-fixed, paraffin embedded (rat stomach) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SMC6) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C