PITX3 polyclonal antibody

Product Specifications

Background

The transcription factor PITX3 is expressed selectively in the midbrain and regulates the differentiation and survival of dopaminergic neurons. Lack of this factor results in a degeneration similar to that seen in Parkinson's disease. PITX3 is also important in eye developement; mutations of the PITX3 gene have been associated with a familial form of cataracts.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

Homeobox protein PITX 3; Homeobox protein PITX3; MGC12766; Paired like homeodomain transcription factor 3; Paired-like homeodomain transcription factor 3; Pituitary homeobox 3; PITX 3; Pitx3; PITX3_HUMAN; PTX 3; PTX3.

Swiss Prot

O75364

Reactivity

Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit

Immunogen

KLH conjugated synthetic peptide derived from human PITX3:231-302/302

Conjugation

Unconjugated

Applications

IHC-P

Dilution

IHC-P=1:100-500 () not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

Purity

Affinity purified by Protein A

Modification

Unmodification

Molecular Weight

~32kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PITX3 polyclonal Antibody detects endogenous levels of PITX3 protein.

Applications Notes

Paraformaldehyde-fixed, paraffin embedded (Mouse brain) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PITX3) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.