Phospho-KLF5 (Ser311) polyclonal antibody

Product Specifications

Background

Kinesin 5B; Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2; BTEB2; intestine enriched krüppel-like factor; IKLF; Kruppel like factor 5.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

KLF5 (phospho Ser311) ; KLF5 (phospho S311) ; Kinesin 5B; Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2; BTEB2; intestine enriched krüppel-like factor; IKLF; Kruppel like factor 5; KLF5_HUMAN.

Swiss Prot

Q13887

Reactivity

Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit

Immunogen

KLH conjugated synthesised phosphopeptide derived from human KLF5 around the phosphorylation site of Ser311:PG (p-S) PD

Conjugation

Unconjugated

Applications

IHC-P

Dilution

IHC-P=1:100-500 () not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

Purity

Affinity purified by Protein A

Modification

Phosphorylation

Molecular Weight

~50kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-KLF5 (Ser311) polyclonal antibody detects endogenous levels of KLF5 protein only when phosphorylated at Ser311.

Applications Notes

Paraformaldehyde-fixed, paraffin embedded (Mouse placenta) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-KLF5 (Ser311) ) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.