Rsk-1 (phospho-S380) polyclonal antibody
Product Specifications
Background
The family of ribosomal S6 kinases (Rsks), designated Rsk-1 (or MAPKAP kinase-1), Rsk-2 and Rsk-3, are intracellular serine/threonine kinases that are important signaling intermediates in response to a broad range of ligand activated receptor tyrosine kinases. A unique feature common to the members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. An additional Rsk protein, Rsk-4, shows a high level of homology to the three previously isolated members of the human Rsk family. Rsk-4 is most abundantly expressed in brain and kidney and plays a role in normal neuronal development.
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Ribosomal protein S6 kinase alpha-1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; Ribosomal S6 kinase 1; RSK-1; RPS6KA1; MAPKAPK1A; RSK1; S6K-α1; S6K α1; S6Kα1
Swiss Prot
Q15418
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human Rsk-1 around the phosphorylation site of Serine 380.
Conjugation
Unconjugated
Related Pathways
Translational Contral elF4 and p70 S6 Kinase, Signaling Pathways Activating p38 MAP KinaseInhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, Cell Cycle G2 M DNA Damage Signaling Pathway, Mitochondrial Control of Apoptosis, MTOR signaling
Applications
WB IHC
Dilution
WB: 1:1000~1:2000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 90 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
P-Rsk-1 (S380) polyclonal antibody detects endogenous levels of Rsk-1 protein only when phosphorylated at Ser380.
Applications Notes
Western blot (WB) analysis of p-Rsk-1 (S380) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:HEK293T treated with EGF (100ng/ml,30 minutes) whole cell lysate (40ug) Lane3:The Spleen tissue lysate of Mouse (40ug) Lane4:The Heart tissue lysate of Rat (40ug)
Host or Source
Rabbit
CAS Number
9007-83-4
Available Sizes
Curated Selection
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