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JAK1 monoclonal antibody

Product Specifications

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors. Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats, Shc, insulin receptor substrates, and focal adhesion kinase (FAK), typically contain SH2 or other phospho-tyrosine-binding domains.

CAS Number

9007-83-4

Structure Composition

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Product Name Alternative

JAK1A; JAK1B; Tyrosine-protein kinase JAK1; Janus kinase 1; JAK-1

Swiss Prot

P23458

Reactivity

Human, Rat

Immunogen

Purified recombinant human Jak1 protein fragments expressed in E.coli.

Conjugation

Unconjugated

Related Pathways

ErbB HER Signaling, Jak Stat : IL-6 Receptor Signaling, Inhibition of Apoptosis, PI3K AKT signaling.

Applications

WB, IF/ICC, IP

Dilution

WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), IP (1/10 - 1/50)

Purity

The antibody was purified by immunogen affinity chromatography.

Modification

Unmodification

Molecular Weight

~ 130 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of JAK1 protein.

Applications Notes

Western blot analysis of JAK1 expression in C6 (A), Ramos (B), Jurkat (C), Hela (D) whole cell lysates.

Host or Source

Mouse

Available Sizes

Curated Selection

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