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Phospho-CaMKII (Thr305) polyclonal antibody

Product Specifications

Background

CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2B; CAM2; CAMK2; CAMKB; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; CAMK2D; CAMKD; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit delta; CaMK-II subunit delta

Swiss Prot

Q9UQM7/Q13554/Q13557

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig, Zebrafish

Immunogen

KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T305 of human CaMKII.

Conjugation

Unconjugated

Applications

WB, IHC

Dilution

WB: 1/500-1/1000 IHC: 1/50-1/100

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Phospho-CaMKII (Thr305) polyclonal antibody detects endogenous levels of CaMKII protein only when phosphorylated at Thr305.

Applications Notes

Western blot analysis of Phospho-CaMKII (Thr305) expression in HEK293T (A), U87MG (B), A549 (C), rat brain (D) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Curated Selection

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