4E-BP1 (Phospho-Ser64) polyclonal antibody

Product Specifications

Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity. Multiple 4E-BP1 residues are phosphorylated in vivo. While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I; EIF4EBP1

Swiss Prot

Q13541

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human 4E-BP1 around the phosphorylation site of Serine 64.

Conjugation

Unconjugated

Related Pathways

PI3K AKT signaling, Hypoxia Signaling, Insulin &Glucose Signaling, MTOR signaling

Applications

WB IP

Dilution

WB: 1:500~1:1000 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 20 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

4E-BP1 (Phospho-Ser64) polyclonal antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at Ser64.

Applications Notes

Western blot (WB) analysis of 4E-BP1 (Phospho-Ser64) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (30ug) Lane2:MCF-7 treated with λ-phosphatase whole cell lysate (30ug)