CD133 polyclonal antibody

Product Specifications

Background

CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells. Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis. Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells. A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Prominin-1; Antigen AC133; Prominin-like protein 1; CD133; PROM1; PROML1; MSTP061

Swiss Prot

O43490

Reactivity

Human

Immunogen

Recombinant protein of human CD133.

Conjugation

Unconjugated

Applications

WB IF

Dilution

WB: 1:1000~1:2000 IF: 1:100~1:500

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodified

Molecular Weight

~ 110 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CD133 polyclonal antibody detects endogenous levels of CD133 protein.

Applications Notes

Western blot (WB) analysis of CD133 polyclonal antibody at 1:1000 dilution Lane1:L02 whole cell lysate (30ug) Lane2:HepG2 whole cell lysate (30ug) Lane3:Panc1 whole cell lysate (30ug) Lane4:H1792 whole cell lysate (30ug)